The average difference in the C T values between all dilutions was 1.07 cycles. Five replicate reactions were run for each dilution using a self-designed TaqMan® assay for IL1R2 and the Rotor-Gene Probe PCR kit on the Rotor-Gene Q. Twofold dilutions of human genomic DNA from 30 ng (10,000 copies) to 0.06 ng (20 copies) were used as a template in real-time PCR. Some are planning to release software upgrades to support HRM analysis. The first multi-well HRM instruments were the Rotor-Gene 6000 (Corbett Life Science) and the LightScanner (Idaho Technology) (PDF). The Rotor-Gene machines support reaction volumes from 5 µl to 100 µl.All pseudo-unknowns were correctly clustered according to genotype.ĭownload Rotor Gene 6000 Software Engineering. A/T polymorphisms (class IV SNPs) are most difficult to discriminate due to minute differences between homozygote alleles (in this example, less than 0.1☌). Data analysis was performed with the unsupervised mode of Rotor-Gene ScreenClust HRM Software. A human A/T SNP in the AHRR7 gene was analyzed using genomic DNA from wild-type (blue), homozygous mutant (green), and heterozygous (red) samples.Įxperiments were performed using the Type-it HRM PCR Kit and a Rotor-Gene Q cycler with an HRM channel.
Is the new operating and analysis software for life science qPCR applications, with a plug-in concept that lets you add new functionality without affecting established workflows.Įxplore the to learn more about the Rotor-Gene Q. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications. QIAGEN's real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands.
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